|Dissolved inorganic and organic nitrogen uptake in the coastal North Sea: a seasonal study|Moneta, A.; Veuger, B.; van Rijswijk, P.; Meysman, F; Soetaert, K.; Middelburg, J.J. (2014). Dissolved inorganic and organic nitrogen uptake in the coastal North Sea: a seasonal study. Est., Coast. and Shelf Sci. 147: 78-86. http://dx.doi.org/10.1016/j.ecss.2014.05.022
In: Estuarine, Coastal and Shelf Science. Academic Press: London; New York. ISSN 0272-7714; e-ISSN 1096-0015, meer
nitrogen uptake; phytoplankton; bacteria; dissolved organic nitrogen; stable isotopes and biomarkers; D-alanine; The Netherlands; Wadden sea; Marsdiep tidal inlet (53.001833? N, 4.789201? E)
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Nitrogen incorporation into total particulate suspended matter, hydrolysable amino acids and bacterial biomarker d-Alanine was assessed seasonally in the coastal North Sea using 15N-labeled ammonium, nitrate, nitrite and 15N- and 13C-labeled urea, glycine, leucine, phenylalanine, and two complex pools of dissolved organic matter (DOM) derived from algal and bacterial cultures (A-DOM, B-DOM). We investigated: 1) uptake rates for the various substrates and their contribution to total N uptake; 2) microbial preferences for the different N sources; 3) the coupling of C and N uptake from organic substrates; 4) the contribution of bacteria to the total microbial uptake of these substrates, and 5) the role of a complex pool of organic matter for plankton nutrition. Seasonality in the preferences for N substrates was observed, with A-DOM and B-DOM being preferred in autumn and winter whereas NH4+ was preferentially taken up in spring and summer. C and N uptake was coupled for all the organic substrates, except urea that was mainly used as a nitrogen source in summer and spring. Bacterial contribution to the uptake of A-DOM and B-DOM was, on an annual average, the lowest among the N-substrates. This suggests an important role for phytoplankton in the incorporation of complex organic matter and the importance of DOM for phytoplankton nutrition.