The Chukchi Sea is an increasing CO₂ sink driven by rapidclimate changes. Understanding the seasonal variation of air-sea CO ₂ exchange and the underlying mechanisms of biogeochemical dynamics is important for predicting impacts of climate change on and feedbacks by the ocean. Here, we present a unique data set of underway sea surface partial pressure of CO₂ (pCO₂) and discrete samples of biogeochemical properties collected in five consecutivecruises in 2014 and examine the seasonal variations in air-sea CO ₂ flux and net community production (NCP). We found that thermaland non-thermal effects have different impacts on sea surface pCO ₂ and thus the air-sea CO₂ flux in different water masses. The Bering summer water combined with meltwater has a significantly greater atmospheric CO₂ uptake potential than that of the Alaskan Coastal Water in the southern Chukchi Sea in summer, due to stronger biological CO₂ removal and a weaker thermal effect. By analyzing the seasonal drawdown of dissolved inorganic carbon (DIC) and nutrients, we found that DIC-based NCP was higher than nitrate-based NCP by 66%–84% and attributable to partially decoupled C and N uptake because of a variable phytoplankton stoichiometry. A box model with a non-Redfield C:N uptake ratio can adequately reproduce observed pCO₂ and DIC, which reveals that, during the intensive growing season (late spring to early summer), 30%–46% CO₂ uptake in the Chukchi Sea was supported by a flexible stoichiometry of phytoplankton. These findings have important ramification for forecasting the responses of CO₂ uptake of the Chukchi ecosystem to climate change.