nieuwe zoekopdracht
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina
On distinguishing between canonical tRNA genes and tRNA gene fragments in prokaryotes
van der Gulik, P.T.S.; Egas, M.; Kraaijeveld, K.; Dombrowski, N.; Groot, A.T.; Spang, A.; Hoff, W.D.; Gallie, J. (2023). On distinguishing between canonical tRNA genes and tRNA gene fragments in prokaryotes. Rna Biology 20: 48-58. https://dx.doi.org/10.1080/15476286.2023.2172370

Bijhorende data:
In: RNA Biology. TAYLOR & FRANCIS INC: Philadelphia. ISSN 1547-6286; e-ISSN 1555-8584, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Author keywords
    tRNA; GtRNAdb; tRNAscanSE; prokaryotes; archaea; thermococcaceae; standard tRNA set
Auteurs  Top 
  • van der Gulik, P.T.S.
  • Egas, M.
  • Kraaijeveld, K.
  • Dombrowski, N., meer
  • Groot, A.T.
  • Spang, A., meer
  • Hoff, W.D.
  • Gallie, J.
Abstract
    Automated genome annotation is essential for extracting biological information from sequence data. The identification and annotation of tRNA genes is frequently performed by the software package tRNAscan-SE, the output of which is listed for selected genomes in the Genomic tRNA database (GtRNAdb). Here, we highlight a pervasive error in prokaryotic tRNA gene sets on GtRNAdb: the mis-categorization of partial, non-canonical tRNA genes as standard, canonical tRNA genes. Firstly, we demonstrate the issue using the tRNA gene sets of 20 organisms from the archaeal taxon Thermococcaceae. According to GtRNAdb, these organisms collectively deviate from the expected set of tRNA genes in 15 instances, including the listing of eleven putative canonical tRNA genes. However, after detailed manual annotation, only one of these eleven remains; the others are either partial, non-canonical tRNA genes resulting from the integration of genetic elements or CRISPR-Cas activity (seven instances), or attributable to ambiguities in input sequences (three instances). Secondly, we show that similar examples of the mis-categorization of predicted tRNA sequences occur throughout the prokaryotic sections of GtRNAdb. While both canonical and non-canonical prokaryotic tRNA gene sequences identified by tRNAscan-SE are biologically interesting, the challenge of reliably distinguishing between them remains. We recommend employing a combination of (i) screening input sequences for the genetic elements typically associated with non-canonical tRNA genes, and ambiguities, (ii) activating the tRNAscan-SE automated pseudogene detection function, and (iii) scrutinizing predicted tRNA genes with low isotype scores. These measures greatly reduce manual annotation efforts, and lead to improved prokaryotic tRNA gene set predictions.
Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs 
IMIS is ontwikkeld en wordt gehost door het VLIZ, voor meer informatie contacteer .