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PsrA positively regulates the unsaturated fatty acid synthesis operon fabAB in Azotobacter vinelandii
Velázquez-Sánchez, C.; Vences-Guzmán, M.Á.; Moreno, S.; Tinoco-Valencia, R.; Espín, G.; Guzmán, J.; Sahonero Canavesi, D.X.; Sohlenkamp, C.; Segura, D. (2021). PsrA positively regulates the unsaturated fatty acid synthesis operon fabAB in Azotobacter vinelandii. Microbiological Research 249: 126775. https://dx.doi.org/10.1016/j.micres.2021.126775
In: Microbiological Research: Jena. ISSN 0944-5013, meer
Peer reviewed article  

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Author keywords
    Azotobacter vinelandii; Transcriptional regulation; Unsaturated fatty acids; Cyclopropane fatty acids

Auteurs  Top 
  • Velázquez-Sánchez, C.
  • Vences-Guzmán, M.Á.
  • Moreno, S.
  • Tinoco-Valencia, R.
  • Espín, G.
  • Guzmán, J.
  • Sahonero Canavesi, D., meer
  • Sohlenkamp, C.
  • Segura, D.

Abstract
    In Pseudomonas spp. PsrA, a transcriptional activator of therpoS gene, regulates fatty acid catabolism by repressing the fadBA5 β-oxidation operon. In Azotobacter vinelandii, a soil bacterium closely related to Pseudomonas species, PsrA is also an activator of rpoS expression, although its participation in the regulation of lipid metabolism has not been analyzed. In this work we found that inactivation of psrA had no effect on the expression of β-oxidation genes in this bacterium, but instead decreased expression of the unsaturated fatty acid biosynthetic operon fabAB (3-hydroxydecanoyl-ACP dehydratase/isomerase and 3-ketoacyl-ACP synthase I). This inactivation also reduced the unsaturated fatty acid content, as revealed by the thin-layer chromatographic analysis, and confirmed by gas chromatography; notably, there was also a lower content of cyclopropane fatty acids, which are synthesized from unsaturated fatty acids. The absence of PsrA has no effect on the growth rate, but showed loss of cell viability during long-term growth, in accordance with the role of these unsaturated and cyclopropane fatty acids in the protection of membranes. Finally, an electrophoretic mobility shift assay revealed specific binding of PsrA to the fabA promoter region, where a putative binding site for this regulator was located. Taken together, our data show that PsrA plays an important role in the regulation of unsaturated fatty acids metabolism in A. vinelandii by positively regulating fabAB.

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