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Methylicorpusculum oleiharenae gen. nov., sp. nov., an aerobic methanotroph isolated from an oil sands tailings pond
Saidi-Mehrabad, A.; Kits, D.K.; Kim, J.-J.; Tamas, I.; Schumann, P.; Khadka, R.; Strilets, T.; Smirnova, A.V.; Rijpstra, W.I.C.; Sinninghe Damsté, J.S; Dunfield, P.F. (2020). Methylicorpusculum oleiharenae gen. nov., sp. nov., an aerobic methanotroph isolated from an oil sands tailings pond. Int. J. Syst. Evol. Microbiol. 70(4): 2499-2508. https://doi.org/10.1099/ijsem.0.004064
In: International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology: Reading. ISSN 1466-5026; e-ISSN 1466-5034, meer
Peer reviewed article  
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Trefwoord
Author keywords
    methanotroph; methylotroph; methane; Methylococcaceae
Auteurs  Top 
  • Saidi-Mehrabad, A.
  • Kits, D.K.
  • Kim, J.-J.
  • Tamas, I.
  • Schumann, P.
  • Khadka, R.
  • Strilets, T.
  • Smirnova, A.V.
  • Rijpstra, W.I.C., meer
  • Sinninghe Damsté, J.S, meer
  • Dunfield, P.F.
Abstract
    An aerobic methane oxidizing bacterium, designated XLMV4T, was isolated from the oxic surface layer of an oil sands tailings pond in Alberta, Canada. Strain XLMV4T is capable of growth on methane and methanol as energy sources. NH4Cl and sodium nitrate are nitrogen sources. Cells are Gram-negative, beige to yellow-pigmented, motile (via a single polar flagellum), short rods 2.0–3.3 µm in length and 1.0–1.6 µm in width. A thick capsule is produced. Surface glycoprotein orcup shape proteins typical of the genera Methylococcus, Methylothermus and Methylomicrobium were not observed. Major isoprenoid quinones are Q-8 and Q-7 at an approximate molar ratio of 71 : 22. Major polar lipids are phosphoglycerol and ornithine lipids. Major fatty acids are C16 : 1 ω8+C16 : 1 ω7 (34 %), C16 : 1 ω5 (16 %), and C18 : 1 ω7 (11 %). Optimum growth is observed at pH 8.0 and 25 °C. The DNA G+C content based on a draft genome sequence is 46.7 mol%. Phylogenetic analysis of 16S rRNA genes and a larger set of conserved genes place strain XLMV4Twithin the class Gammaproteobacteria and familyMethylococcaceae , most closely related to members of the genera Methylomicrobium and Methylobacter (95.0–97.1 % 16S rRNA gene sequence identity). In silico genomic predictions of DNA–DNA hybridization values of strain XLMV4T to the nearest phylogenetic neighbours were all below 26 %. On the basis of the data presented, strain XLMV4T is considered to represent a new genus and species for which the name Methylicorpusculum oleiharenae is proposed. Strain XLMV4T (=DSMZ DSM 27269=ATCC TSD-186) is the type strain.
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