nieuwe zoekopdracht
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Physiological, chemotaxonomic and genomic characterization of two novel piezotolerant bacteria of the family Marinifilaceae isolated from sulfidic waters of the Black Sea
Yadav, S.; Villanueva, L.; Bale, N.; Koenen, M.; Hopmans, E.C.; Sinninghe Damsté, J.S (2020). Physiological, chemotaxonomic and genomic characterization of two novel piezotolerant bacteria of the family Marinifilaceae isolated from sulfidic waters of the Black Sea. Syst. Appl. Microbiol. 43(5): 126122. https://doi.org/10.1016/j.syapm.2020.126122
In: Systematic and Applied Microbiology. Elsevier: Jena. ISSN 0723-2020; e-ISSN 1618-0984, meer
Peer reviewed article  

Beschikbaar in  Auteurs 
    NIOZ: NIOZ Open Repository 354998

Author keywords
    Marinifilaceae; Ancylomarina euxinussp. nov.; Labilibaculum euxinussp. nov.; Black Sea

Auteurs  Top 
  • Koenen, M., meer
  • Hopmans, E.C., meer
  • Sinninghe Damsté, J.S, meer

Abstract
    Diversity analyses of microbial enrichments obtained from deep sulfidic water (2000 m) collected from the Black Sea indicated the presence ofeleven novel putative lineages of bacteria affiliated to the family Marinifilaceae of the phylum Bacteroidetes. Pure cultureswere obtained for four strains (i.e. M1PT, M3P, A4 T and 44) of this family, which could be grouped into two different clades based on their 16S rRNA gene sequences. All four strains were Gram-negative, rod-shaped and facultative anaerobic bacteria. The genomes of all strains were sequenced and physiological analyses were performed. All strains utilized a wide range of carbon sources, which was supported by the presence of the pathways involved in carbon utilization encoded by their genomes. The strains were able to grow at elevated hydrostatic pressure (up to 50 MPa), which coincided with increased production of unsaturated and branched fatty acids, and a decrease in hydroxy fatty acids. Intact polar lipid analysis of all four strains showed the production of ornithine lipids, phosphatidylethanolamines and capnine lipids as major intact polar lipids (IPLs). Genes involved in hopanoid biosynthesis were also identified. However, bacteriohopanepolyols (BHPs) were not detected in the strains. Based on distinct physiological, chemotaxonomic, genotypic and phylogenetic differences compared to other members of the genera Ancylomarina and Labilibaculum, it was concluded that strains M1PT and A4T represented twonovel species for which the names Ancylomarina euxinus sp. nov. and Labilibaculum euxinus sp. nov., respectively, are proposed.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs 
IMIS is ontwikkeld en wordt gehost door het VLIZ, voor meer informatie contacteer .