nieuwe zoekopdracht
[ meld een fout in dit record ]mandje (1): toevoegen | toon Print deze pagina

one publication added to basket [320668]
A packing for A-form DNA in an icosahedral virus
Wang, F.; Liu, Y.; Su; Osinski, T.; de Oliveira; Conway; Schouten, S.; Krupovic, M.; Prangishvili, D.; Egelman, E.H. (2019). A packing for A-form DNA in an icosahedral virus. Proc. Natl. Acad. Sci. U.S.A. 116(45): 22591-22597.
In: Proceedings of the National Academy of Sciences of the United States of America. The Academy: Washington, D.C.. ISSN 0027-8424; e-ISSN 1091-6490, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

Author keywords
    DNA structure; hyperthermophilic acidophiles; cryo-EM

Auteurs  Top 
  • Wang, F.
  • Liu, Y.
  • Su, Z.
  • Osinski, T.
  • de Oliveira, G.A.P.
  • Conway, J.F.
  • Schouten, S., meer
  • Krupovic, M.
  • Prangishvili, D.
  • Egelman, E.H.

    Studies on viruses infecting archaea living in the most extreme environments continue to show a remarkable diversity of structures, suggesting that the sampling continues to be very sparse. We have used electron cryo-microscopy to study at 3.7-Å resolution the structure of the polyhedral virus 1 (SPV1), which was originally isolated from a hot, acidic spring in Beppu, Japan. The 2 capsid proteins with variant single jelly-roll folds form pentamers and hexamers which assemble into a T = 43 icosahedral shell. In contrast to tailed icosahedral double-stranded DNA (dsDNA) viruses infecting bacteria and archaea, and herpesviruses infecting animals and humans, where naked DNA is packed under very high pressure due to the repulsion between adjacent layers of DNA, the circular dsDNA in SPV1 is fully covered with a viral protein forming a nucleoprotein filament with attractive interactions between layers. Most strikingly, we have been able to show that the DNA is in an A-form, as it is in the filamentous viruses infecting hyperthermophilic acidophiles. Previous studies have suggested that DNA is in the B-form in bacteriophages, and our study is a direct visualization of the structure of DNA in an icosahedral virus.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs 
IMIS is ontwikkeld en wordt gehost door het VLIZ, voor meer informatie contacteer .