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Qualitative growth potential test for brackish Vaucheria species
van Belzen, J. (2018). Qualitative growth potential test for brackish Vaucheria species. Protocol Exchange april 2018. https://dx.doi.org/10.1038/protex.2018.041
In: Protocol Exchange. Nature Publishing Group: London. ISSN 2043-0116, meer

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Author keywords
    algae; culture; growth potential; sediment; Vaucheria

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  • van Belzen, J., meer

Abstract
    The yellow-green algae Vaucheria can be found on tidal flats as densely interwoven mats. Such dense mats can stabilise sediments and result in a ridge-and-runnel bedform. While Vaucheria is visually covering the ridges as thick algal mats, the runnels are apparently deprived of the filamentous algae. In the present protocol I describe the procedure for sampling and testing sediments for the presence of Vaucheria propagules. The method provides a qualitative test for the presence of algae propagules of Vaucheria in bare sediments but is non-exclusive as also other algae (e.g., microphytobenthos) will grow when present. To check for cross-contamination and sufficient growth conditions, appropriate negative and positive controls are implemented.

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