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A method for quantifying heterocyst glycolipids in biomass and sediments
Bale, N.; de Vries, S.; Hopmans, E.C.; Sinninghe Damsté, J.S.; Schouten, S. (2017). A method for quantifying heterocyst glycolipids in biomass and sediments. Org. Geochem. 110: 33-35. https://dx.doi.org/10.1016/j.orggeochem.2017.04.010
In: Organic Geochemistry. Elsevier: Oxford; New York. ISSN 0146-6380, meer
Peer reviewed article  

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Trefwoord
Author keywords
    Heterocyst; Cyanobacteria; Heterocyst glycolipids; Semi-preparative high performance liquid chromatography; Quantification

Auteurs  Top 
  • Bale, N., meer
  • de Vries, S.
  • Hopmans, E.C., meer
  • Sinninghe Damsté, J.S., meer
  • Schouten, S., meer

Abstract
    Heterocyst glycolipids (HGs) are biomarkers for heterocystous N2-fixing cyanobacteria and consist of a sugar moiety bound to an even numbered C26–C32 alkyl chain, containing various alcohol or ketone groups. They have been reported in ancient sediments but, due to a lack of standards, they were quantified in terms of integrated peak area response. Here we describe the use of a commercially available glycolipid (n-dodecyl-β-d-glucopyranoside) as an internal standard (IS) for reliable quantitative analysis. The common HG 1-(O-hexose)-3,25-hexacosanediol was isolated using both normal and reversed phase chromatography and used to assess the relative response factor compared with the IS, allowing correction of difference in mass spectral response between the IS and the target HGs.

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