nieuwe zoekopdracht
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Isolation of cyanophage CrV infecting Cylindrospermopsis raciborskii and the influence of temperature and irradiance on CrV proliferation
Steenhauer, L.M.; Wierenga, J.; Carreira, C; Limpens, R.W.A.L.; Koster, A.J.; Pollard, P.C.; Brussaard, C.P.D. (2016). Isolation of cyanophage CrV infecting Cylindrospermopsis raciborskii and the influence of temperature and irradiance on CrV proliferation . Aquat. Microb. Ecol. 78: 11-23. dx.doi.org/10.3354/ame01800
In: Aquatic Microbial Ecology. Inter-Research: Oldendorf/Luhe. ISSN 0948-3055, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

Trefwoord
Author keywords
    Invasive cyanobacteria; Cyanophage; Freshwater; Latent period; Burst size; Temperature; Light

Auteurs  Top 
  • Steenhauer, L.M.
  • Wierenga, J.
  • Carreira, C, meer
  • Limpens, R.W.A.L.
  • Koster, A.J.
  • Pollard, P.C.
  • Brussaard, C.P.D., meer

Abstract
    ABSTRACT: A lytic virus that infects a European strain of the freshwater filamentous cyano-bacterium Cylindrospermopsis raciborskii was isolated from a lake in the Netherlands and partially characterised. With a genome size of 110 ± 15 kb, an icosahedral capsid of 65 ± 1 nm (n = 22) and a long non-contractile tail of 612 ± 31 nm (n = 15), this dsDNA cyanophage CrV appears to belong to the Siphoviridae family. CrV was highly host specific, not infecting other filamentous cyanobacteria species isolated from the same lake, nor 4 Australian strains of C. raciborskii. The latent period of this cyanophage was 20-24 h. Varying the irradiance affected cyanophage-host interactions: at low light (20 µmol quanta m-2 s-1) the latent period was 1.3 times longer compared with at mid light (90 µmol quanta m-2 s-1); burst size at mid light was 332 CrV per lysed host cell, at low light it was halved (48%) and at high light (250 µmol quanta m-2 s-1) the burst size was further reduced to only 14% of that of mid light. Temperature also affected the virus growth characteristics: the CrV latent period at high temperature (30°C) was reduced to just 11% (compared with a mid temperature of 22°C), but still the burst size increased to 541 CrV per lysed host cell; at low temperature (15°C) the latent period was prolonged 1.3-fold and the burst size was reduced to 43%. Our findings indicate that ecologically relevant environmental factors can affect the extent of viral lysis of C. raciborskii, advancing our understanding of the spread of this invasive cyanobacterium across Europe.

Alle informatie in IMIS valt onder het VLIZ Privacy beleid Top | Auteurs 
IMIS is ontwikkeld en wordt gehost door het VLIZ, voor meer informatie contacteer .