nieuwe zoekopdracht
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Protein folding at extreme temperatures: current issues
Feller, G. (2018). Protein folding at extreme temperatures: current issues. Seminars in Cell & Developmental Biology 84: 129-137.
In: Seminars in Cell & Developmental Biology. ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD: London. ISSN 1084-9521
Peer reviewed article  

Beschikbaar in  Auteur 

Author keywords
    Protein folding; Extremophiles; Trigger factor; Prolyl isomerization;Thermodynamic stability

Auteur  Top 
  • Feller, G.

    The range of temperatures compatible with life is currently estimated from −25 °C, as exemplified by metabolically active bacteria between sea ice crystals, and up to 122 °C in hydrothermal vents as exemplified by the archaeon Methanopyrus kandleri. In the context of protein folding, as soon as a polypeptide emerges from the ribosome, it is exposed to the effects of environmental temperatures. Recent investigations have shown that the rate of protein folding is not adapted to extreme temperatures and should be very fast at high temperature and low in cold environments. This lack of adaptation is driven by kinetic constraints on protein stability. To counteract the deleterious effects of fast protein folding in hyperthermophiles, chaperones such as the Trigger Factor hold and slow down the rate of folding intermediates. Prolyl isomerization, a rate-limiting step in the folding of many proteins, is strongly temperature-dependent and impairs folding of psychrophilic proteins in the cold. This is compensated by reduction of the proline content in cold-adapted proteins, by an increased number of prolyl isomerases encoded in the genome of psychrophilic microorganisms and by overexpression of prolyl isomerases under low temperature cultivation. After folding, the native state is reached and although extremophilic proteins share the same fold, dramatic differences in stability have been recorded by differential scanning calorimetry.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteur 
IMIS is ontwikkeld en wordt gehost door het VLIZ, voor meer informatie contacteer .