nieuwe zoekopdracht
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Fish peripheral blood mononuclear cells preparation for future monitoring applications
Pierrard, M.-A.; Roland, K.; Kestemont, P.; Dieu, M.; Raes, M.; Silvestre, F. (2012). Fish peripheral blood mononuclear cells preparation for future monitoring applications. Analytical Biochemistry 426(2): 153-165. https://hdl.handle.net/10.1016/j.ab.2012.04.009
In: Analytical Biochemistry. Academic Press: San Diego, CA,. ISSN 0003-2697; e-ISSN 1096-0309
Peer reviewed article  

Beschikbaar in  Auteurs 

Trefwoorden
    Pisces [WoRMS]
    Marien; Brak water; Zoet water
Author keywords
    PBMCs; Postnuclear fraction; Flow cytometry; Fish; MTS; Subproteomics

Auteurs  Top 
  • Pierrard, M.-A.
  • Roland, K.
  • Kestemont, P.
  • Dieu, M.
  • Raes, M.
  • Silvestre, F.

Abstract
    Fish species possess many specific characteristics that support their use in ecotoxicology. Widely used in clinical research, peripheral blood mononuclear cells (PBMCs) can reasonably be exploited as relevant target cells in the assessment of environmental chemical toxicity. The current article focuses on the methods necessary to isolate, characterize, and culture fish PBMCs. These procedures were successfully applied on an endangered species, the European eel (Anguilla anguilla L.), and on an economically important and worldwide exported species, the Asian catfish (Pangasianodon hypophthalmus S.). Proteomic approaches can be useful to screen xenobiotic exposure at the protein expression level, giving the opportunity to develop early warning signals thanks to molecular signatures of toxicity. To date, a major limitation of proteomic analyses is that most protein expression profiles often reveal the same predominant and frequently differentially expressed families of proteins regardless of the experimental stressing conditions. The current study describes a methodology to get a postnuclear fraction of high quality isolated from fish PBMCs in order to perform subsequent subproteomic analyses. Applied on samples from eel, the subproteomic analysis (two-dimensional differential in-gel electrophoresis) allowed the identification by liquid chromatography–tandem mass spectrometry and searches in the full NCBInr (National Center for Biotechnology Information nonredundant) database of 66 proteins representing 36 different proteins validated through Peptide and Protein Prophet of Scaffold software.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs 
IMIS is ontwikkeld en wordt gehost door het VLIZ, voor meer informatie contacteer .