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A CRISPR/Cas9 system adapted for gene editing in marine algae
Nymark, M.; Sharma, A.K.; Sparstad, T.; Bones, A.M.; Winge, P. (2016). A CRISPR/Cas9 system adapted for gene editing in marine algae. NPG Scientific Reports 6(24951): 6 pp. http://hdl.handle.net/10.1038/srep24951
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322; e-ISSN 2045-2322, meer
Peer reviewed article  

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Auteurs  Top 
  • Nymark, M.
  • Sharma, A.K.
  • Sparstad, T.
  • Bones, A.M.
  • Winge, P.

Abstract
    Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.

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