nieuwe zoekopdracht
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Atypical reactive center Kunitz-type inhibitor from the sea anemone Heteractis crispa
Gladkikh, I.; Monastyrnaya, M.; Leychenko, E.; Zelepuga, E.; Chausova, V.; Isaeva, M.; Anastyuk, S.; Andreev, Y.; Peigneur, S.; Tytgat, J.; Kozlovkaya, E. (2012). Atypical reactive center Kunitz-type inhibitor from the sea anemone Heteractis crispa. Mar. Drugs 10(7): 1545-1565. hdl.handle.net/10.3390/md10071545
In: Marine Drugs. Molecular Diversity Preservation International (MDPI): Basel. ISSN 1660-3397; e-ISSN 1660-3397, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

Trefwoorden
    Heteractis crispa (Hemprich & Ehrenberg in Ehrenberg, 1834) [WoRMS]
    Marien
Author keywords
    sea anemone; Kunitz-type protease inhibitor; structure; function; channels

Auteurs  Top 
  • Gladkikh, I.
  • Monastyrnaya, M.
  • Leychenko, E.
  • Zelepuga, E.
  • Chausova, V.
  • Isaeva, M.
  • Anastyuk, S.
  • Andreev, Y.
  • Peigneur, S.
  • Tytgat, J.
  • Kozlovkaya, E.

Abstract
    The primary structure of a new Kunitz-type protease inhibitor InhVJ from the sea anemone Heteractis crispa (Radianthus macrodactylus) was determined by protein sequencing and cDNA cloning. InhVJ amino acid sequence was shown to share high sequence identity (up to 98%) with the other known Kunitz-type sea anemones sequences. It was determined that the P1 Thr at the reactive site resulted in a decrease of the Ki of InhVJ to trypsin and a-chymotrypsin (7.38 × 10-8 M and 9.93 × 10-7 M, respectively). By structure modeling the functional importance of amino acids at the reactive site as well as at the weak contact site were determined. The significant role of Glu45 for the orientation and stabilization of the InhVJ-trypsin complex was elucidated. We can suggest that there has been an adaptive evolution of the P1 residue at the inhibitor reactive site providing specialization or functional diversification of the paralogs. The appearance of a key so-called P1 Thr residue instead of Lys might lead to refinement of inhibitor specificity in the direction of subfamilies of serine proteases. The absence of Kv channel and TRPV1-receptor modulation activity was confirmed by electrophysiological screening tests.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs 
IMIS is ontwikkeld en wordt gehost door het VLIZ, voor meer informatie contacteer .