|A rapid PCR–RFLP method for the identification of Lophius species|Armani, A.; Castigliego, L.; Tinacci, L.; Gandini, G.; Gianfaldoni, D.; Guidi, A. (2012). A rapid PCR–RFLP method for the identification of Lophius species. Eur. Food Res. Technol. 235(2): 253-263. http://dx.doi.org/10.1007/s00217-012-1754-3
In: European Food Research and Technology. Springer-Verlag: Heidelberg; Berlin. ISSN 1438-2377; e-ISSN 1438-2385
Identificatie; PCR; Soorten; Lophius Linnaeus, 1758 [WoRMS]; Marien
PCR–RFLP; Cytochrome b; Species identification
|Auteurs|| || Top |
- Armani, A.
- Castigliego, L.
- Tinacci, L.
- Gandini, G.
- Gianfaldoni, D.
- Guidi, A.
The seven Anglerfish species, which belong to the genus Lophius, have a different value on the market, worldwide. If whole fishes can be identified by their morphological characteristics, they become indistinguishable when prepared or processed. In this study, a rapid method based on polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) was developed for the authentication of the seven Lophius species, using a cytochrome b gene fragment of 566 bp. After a genus-specific PCR, a fast digestion with the restriction enzyme BfaI, followed by agarose gel electrophoresis, allowed a clear species identification by producing specific restriction patterns. The total time required was as low as 6 h, DNA extraction included. The method was then used to analyse 48 commercial samples, whose phylogenetic analysis confirmed the PCR–RFLP response at 100 %. Results showed that mislabelling occurs on the market regardless the kind of processing.