|Preparation of fish tissues for electron microscopy|
In: Journal of Electron Microscopy Technique. John Wiley & Sons: New York. ISSN 0741-0581; e-ISSN 1553-0817
Analytical techniques > Microscopy > Electron microscopy
Fauna > Aquatic organisms > Aquatic animals > Fish
Many of the techniques applied to study the morphology of lower vertebrates were originally developed for analysis of mammalian tissues. However, not all of these methods, especially those involving tissue perfusion, can be directly applied to piscine tissues because of the considerable physiological differences between fish and mammals. The present paper examines these physiological variables in fish and describes how they might affect morphological studies that employ tissue perfusion. A method for perfusion of fish tissues is described in detail. This procedure can be used for administration of fixatives, chemical markers, and vascular replicating resins.