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Untangling genomes from metagenomes: revealing an uncultured class of marine Euryarchaeota
Iverson, V.; Morris, R.M.; Frazar, C.D.; Berthiaume, C.T.; Morales, R.L.; Armbrust, E.V. (2012). Untangling genomes from metagenomes: revealing an uncultured class of marine Euryarchaeota. Science (Wash.) 335(6068): 587-590 + supporting online materials. http://dx.doi.org/10.1126/science.1212665
In: Science (Washington). American Association for the Advancement of Science: New York, N.Y. ISSN 0036-8075; e-ISSN 1095-9203, meer
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Auteurs  Top 
  • Iverson, V.
  • Morris, R.M.
  • Frazar, C.D.
  • Berthiaume, C.T.
  • Morales, R.L.
  • Armbrust, E.V.

Abstract
    Ecosystems are shaped by complex communities of mostly unculturable microbes. Metagenomes provide a fragmented view of such communities, but the ecosystem functions of major groups of organisms remain mysterious. To better characterize members of these communities, we developed methods to reconstruct genomes directly from mate-paired short-read metagenomes. We closed a genome representing the as-yet uncultured marine group II Euryarchaeota, assembled de novo from 1.7% of a metagenome sequenced from surface seawater. The genome describes a motile, photo-heterotrophic cell focused on degradation of protein and lipids and clarifies the origin of proteorhodopsin. It also demonstrates that high-coverage mate-paired sequence can overcome assembly difficulties caused by interstrain variation in complex microbial communities, enabling inference of ecosystem functions for uncultured members.

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