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Quantitative and qualitative analysis of retinoids in Artemia and copepods by HPLC and diode array detection
Moren, M.; Gundersen, T.E.; Hamre, K. (2005). Quantitative and qualitative analysis of retinoids in Artemia and copepods by HPLC and diode array detection. Aquaculture 246(1-4): 359-365. dx.doi.org/10.1016/j.aquaculture.2005.01.017
In: Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo. ISSN 0044-8486; e-ISSN 1873-5622
Peer reviewed article  

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Author keywords
    Artemia; copepods; dioarray; HPLC; retinal; retinoic acid; retinol;

Auteurs  Top 
  • Moren, M.
  • Gundersen, T.E.
  • Hamre, K.

Abstract
    Different concentrations of retinoids have been reported in Artemia. The levels vary from 0-362 µg g-1 Artemia (dry wt.). The aim of this study was to investigate the actual retinoid concentration in Artemia and compare it with levels in copepods. Samples of both DC-DHA Selco (INVE, Ghent, Belgium) from the bottle and after 12 h in the enrichment tank in addition to enriched and unenriched Artemia and copepods from a pond system were collected and analysed for vitamin A components. The analyses were conducted using two different HPLC (high pressure liquid chromatography) methods to ensure the justness of the results. The DC-DHA Selco contained 132.7 µg vitamin A g-1. After 12 h in the enrichment tank we could not detect any vitamin A. None of the following retinoids were observed in Artemia or copepods with any of the methods: all-trans-retinol (at-ROH), 9-cis-ROH or at-3,4-didehydro-ROH (at-3,4-dd-ROH). Method 2 detects several other retinoids when present, but none of the following were found in the two live prey organisms: cis or trans 3,4-dd-RA, cis or trans 3,4-dd-ROH, cis or trans 3,4-dd-RAL, cis or trans ROH or cis or trans RA. This implies that larvae feeding on either copepods or Artemia must meet their retinoid requirement from other sources than retinoids.

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