nieuwe zoekopdracht
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Microsatellite DNA marker development and genetic diversity of Branchiostoma belcheri in Xiamen waters
Liu, W.-y.; Zhong, J.; Xu, W.; Wang, Y.-Q. (2011). Microsatellite DNA marker development and genetic diversity of Branchiostoma belcheri in Xiamen waters. Mar. Biol. Res. 7(8): 826-861.
In: Marine Biology Research. Taylor & Francis: Oslo; Basingstoke. ISSN 1745-1000; e-ISSN 1745-1019, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

    Acids > Organic compounds > Organic acids > Nucleic acids > DNA
    Genetic diversity
    Branchiostoma belcheri tsingtauense Tchang-Shi & Koo, 1936 [WoRMS]
Author keywords
    Amphioxus; Branchiostoma belcheri; DNA molecular marker; geneticdiversity; microsatellite DNA

Auteurs  Top 
  • Liu, W.-y.
  • Zhong, J.
  • Xu, W.
  • Wang, Y.-Q.

    Amphioxus occupies a transitional position between invertebrates and vertebrates and is therefore a promising laboratory model animal. As a powerful molecular marker in population genetics studies, microsatellite DNA is required for both the investigation of genetic diversity of a wild amphioxus population and strain breeding of laboratory cultured amphioxus. In the present study, we constructed a microsatellite-enriched DNA library of amphioxus Branchiostoma belcheri to develop this molecular marker. After screening the library and combining it with the data obtained from a public database, we successfully developed 21 pair of primers that could effectively amplify the microsatellite loci of amphioxus. Of those primer pairs, seven were in deviation of Hardy–Weinberg equilibrium, mostly caused by null alleles. The others had no linkage disequilibrium, indicating they are applicable for the genetic studies. Using those primer pairs, we further detected the genetic diversity of the wild amphioxus population in Xiamen waters. The results revealed that the number of alleles of each microsatellite locus was between three and 18 in the population, and observed and expected heterozygosities of the loci ranged from 0.415 to 0.947 and from 0.300 to 1.000, indicating very high genetic diversity of the population.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs 
IMIS is ontwikkeld en wordt gehost door het VLIZ, voor meer informatie contacteer .