nieuwe zoekopdracht
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Chemical composition of Skeletonema costatum (Grev.) Cleve and Paplova (Monochrysis) lutheri (Droop) Green as a function of nitrate-, phosphate-, and iron-limited growth
Sakshaug, E.; Holm-Hansen, O. (1977). Chemical composition of Skeletonema costatum (Grev.) Cleve and Paplova (Monochrysis) lutheri (Droop) Green as a function of nitrate-, phosphate-, and iron-limited growth. J. Exp. Mar. Biol. Ecol. 29(1): 1-34
In: Journal of Experimental Marine Biology and Ecology. Elsevier: New York. ISSN 0022-0981; e-ISSN 1879-1697, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

Trefwoorden
    Chemische samenstelling; Limiterende factoren; Pavlova lutheri (Droop) J.C.Green, 1975 [WoRMS]; Skeletonema costatum (Greville) Cleve, 1873 [WoRMS]; Marien

Auteurs  Top 
  • Sakshaug, E.
  • Holm-Hansen, O.

Abstract
    The variation in chemical composition of S. costatum and P. lutheri was studied in batch cultures with N-, P-, and Fe-deficient media under continuous light. In vivo fluorescence, chlorophyll a, ATP, cellular nitrogen, carbon, and phosphorus, and cell density were measured. The exponential growth rate was 2 . 9-3 . 5 doubl. Day-1 for Skeletonema and 1 . 2-1 . 9 doubl. Day-1 for Pavlova, with the lower rates pertaining to cultures in Fe-deficient media. In exponentially growing cultures nitrogen and carbon per cell increased with an increasing N/P ratio of the media, whereas chlorophyll a, ATP, and organic phosphorus per cell remained relatively constant. In Fe-deficient cells nitrogen and carbon per cell was high, as for cells in media with a high N/P ratio. The total variation in exponential cells was contained within a factor of three. Larger differences in chemical composition were found between exponentially growing cells and nutrient deficient cells. For all types of nutrient deficiency the chl/N and chl/C ratios decreased when cells went from the exponential phase to the starvation phase. The ratio between in vivo fluorescence and chlorophyll a increased 4-5 fold. The C/N ratio (atomic) of both species increased from 5-7 . 5 for exponentially growing cells up to 35 during N-deficiency and up to 13 during P-deficiency. For cells in Fe-deficient medium values were scattered in the 7-11 range. ATP and organic phosphorus followed each other closely and had values which were about 3 times higher in Skeletonema than in Pavlova when using any other parameter as a frame of reference for identical media and growth stages. In N-deficient media the N/ATP ratio was virtually the same for exponentially growing cells and starved cells. The C/ATP ratio reached high levels in nutrient deficient cells, with a maximum of 9000 for P-deficient cells of Pavlova. Both species had a minimum value of polyphosphates corresponding to about 10% of the total phosphorus. In P-rich media Pavlova had storage polyphosphates up to 70% of the total P. Storage polyphosphates were not observed in Skeletonema. With the set of factors used it is possible to identify growth stages and type of nutrient deficiency for a given culture. It is indicated that, with certain limitations, this approach may be applicable in field studies.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs 
IMIS is ontwikkeld en wordt gehost door het VLIZ, voor meer informatie contacteer .