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Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)
Pujolar, J.M.; Maes, G.E.; Van Houdt, J.K.J.; Zane, L. (2009). Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla). Mol. Ecol. Resour. 9(1): 233-235. dx.doi.org/10.1111/j.1755-0998.2008.02419.x
In: Molecular Ecology Resources. Blackwell Publishing: Oxford. ISSN 1755-098X; e-ISSN 1755-0998, meer
Peer reviewed article  

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Trefwoorden
    Microsatellites
    Sequences
    Anguilla anguilla (Linnaeus, 1758) [WoRMS]
    Marien
Author keywords
    Anguilla; EST; European eel; microsatellite

Auteurs  Top 
  • Pujolar, J.M.
  • Maes, G.E.
  • Van Houdt, J.K.J.
  • Zane, L.

Abstract
    A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel (Anguilla rostrata).

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